It is widely recognized that mycotoxins in feed ingredients pose a significant threat to pig health and performance. In fact, of all livestock species, pigs are considered to be the most susceptible to the negative effects of mycotoxins, with the Fusarium-derived mycotoxins fumonisin (FUM) and zearalenone (ZON) known to be acutely toxic to pigs.

Yet, until recently, the impact of species-specific aspects of the mycotoxin threat and potential solutions has been largely overlooked. As a result, the development of in-feed adsorbents and deactivators has focused primarily on a one-size-fits-all strategy. This is now changing, however, following the emergence of a strong rationale for mycotoxin solutions that target the specific mycotoxin threat and physiological differences of each individual species.

Differing mycotoxin priorities

For pigs, the greatest risk to health and performance comes from the various mycotoxins produced by a Fusarium spp. infection of feed ingredients, namely FUM and ZON, plus the trichothecenes deoxynivalenol (DON) and T2 toxin. Aflatoxins produced by Aspergillus spp. are also important, and the clear differences in relative toxicity compared to other species can be seen in Table 1.

relative toxicity of different mycotoxins

There are clear differences in the relative toxicity of different mycotoxins for swine, poultry and ruminants.

Each mycotoxin poses a range of different threats to the pig (Figure 1), with pulmonary oedema characteristic of acute poisoning with FUM, while the trichothecenes are toxic to gut epithelial cells and known to cause intestinal lesions. Since gut health and integrity are critical to efficient nutrient digestion and absorption, such exposure leads to poorer feed conversion efficiency, lower feed intakes and reduced liveweight gain. Along with FUM, the trichothecenes also impair immune function, increasing susceptibility to disease.

negative effects of different mycotoxins

Each mycotoxin poses a range of different threats to the pig. 

Reduced pig performance

In contrast, ZON is the mycotoxin most commonly implicated in reproductive failure, including reduced conception rates — ZON mimics oestrogen — and in extreme cases, embryonic loss. Particularly noticeable in second parity sows, which make up approximately 19 percent of the reproductive herd, ZON exposure can exacerbate the delayed return to oestrus and smaller litter size that typically follows the stress of first lactation, further reducing productivity.

Aflatoxins are important for their effects on both productivity and health. Acting at the cellular level, aflatoxins bind to DNA and prevent RNA synthesis, reducing protein synthesis and cutting growth rates by up to 3.9 percent for every 1 ppm increase.

Posing greatest risk in tropical regions where warm, humid conditions encourage the growth of Aspergillus spp. molds during feed transport and storage, aflatoxins have also been implicated as carcinogens. Any potential residue in muscle tissues is therefore important from a human health perspective, while transfer to the milk in lactating sows has been reported to reduce immune function in piglets.

Negative commercial impact

It is these younger pigs that are at greatest risk. A meta-analysis of over 85 published studies revealed age and total mycotoxin exposure as the two factors having greatest influence on the growth rate effects of mycotoxins. Young animals were found to be most susceptible, with male pigs more affected than females.

Although risk decreases with age and so is clearly linked to stage of production, in-feed mycotoxins remain a threat throughout the pig’s productive life. The negative effects on growth, reproduction and disease susceptibility have the capacity to substantially reduce overall production efficiency and profitability even at low, chronic levels of contamination.

On most commercial pig units, however, total mycotoxin load is relatively high, due primarily to the heavy reliance on cereal grains in pig feeds. The Fusarium spp. fungal diseases prevalent in those cereal crops produce high levels of contamination with FUM, ZON and DON in particular.

In-feed mycotoxin load

Of these, DON is the mycotoxin most frequently found in pig feed, although individual mycotoxins are rarely found in isolation. In 2014 and 2015, Micron Bio-Systems carried out an analysis of common pig feed ingredient samples from across Europe and the Middle East — wheat, wheatfeed, maize, distillers grains — and found DON, FUM and ZON in 66 percent, 56 percent and 53 percent of samples respectively. Maize contained mycotoxins most frequently and at the highest levels.


Overall, nearly half the samples contained a total mycotoxin load classed as high risk (over 500 ppb, Figure 2a). Similar results have been reported in pig feed analysed through the Mycocheck mycotoxin testing service (Figure 2b).

proportion of feed ingredients and swine compound feed

Nearly half the samples contained a total mycotoxin load classed as high risk (over 500 ppb, Figure 2a). Similar results have been reported in pig feed analysed through the Mycocheck mycotoxin testing service (Figure 2b).

Pig diets also rely heavily on human-edible ingredients, and those batches failing to meet the more stringent mycotoxin limits for human consumption often find their way into animal feed. In addition, pigs are regularly fed co-products from the human food chain, where processing can concentrate the mycotoxin load.

Three key strategies

As awareness of the scale of the mycotoxin threat has grown, so too has the use of in-feed mycotoxin deactivators and binders. Designed to bind, transform or degrade mycotoxins, there is a wide range of such products available commercially.

Binding (adsorption) is the most common approach within the feed industry, with clay minerals such as bentonite used to bind with polar mycotoxins (aflatoxin) and yeast cell walls demonstrating some efficacy against non-polar Fusarium spp. mycotoxins. The ability to maintain efficacy within the pH range typically found in the pig’s gastro-intestinal tract (pH 3-7) is critical to success.

An additional strategy, particularly for non-polar mycotoxins, is to remove or modify a particular functional site on the surface of the mycotoxin. This transformation can render the mycotoxin harmless, or expose the binding site of the molecule to mineral binding agents, and is typically achieved using components targeted at either pH 3 or pH 7.

Finally, degradation is the application of multiple transformations to ensure that any mycotoxin fragments remaining after transformation — even if bound to a mineral binder — do not retain any toxic effect. For swine producers, the strategy of transformation and degradation is particularly pertinent, since it is the most effective in eliminating the effects of DON, which is both highly prevalent in pig feeds and acutely toxic to pigs.

Species-specific solutions

The most effective approach is typically a combination of all three strategies, and it is clear from the information above that it is the specific nature of the mycotoxin threat that will dictate how such strategies should be prioritized. It also highlights just how important it is to understand the differences between species, both in terms of exposure and vulnerability.

In pigs, for example, ZON is metabolized to the more toxic alpha-zearalenol, whereas in poultry it is the less toxic beta-zearalenol that is produced instead, and such differences are already recognized by key regulatory authorities. In the EU, maximum permitted limits for FUM and ZON in pig feed are set at 5 ppm and 0.1 ppm, respectively, whereas in poultry the limit for FUM is 20 ppm and there is no specific limit for ZON.

The recent development of species-specific in-feed mycotoxin solutions therefore represents a major advance in mycotoxin remediation, and is already leading the way towards potential customized, bespoke solutions based on individual farm mycotoxin profiles. Although reliant on effective and timely testing of feed samples for actual mycotoxin loading, the potential benefits from such a flexible, yet highly targeted, approach are likely to generate considerable interest in years to come.