Authorities ordered the culling of a poultry flock in Romania in which two birds died of avian flu, and they established a two-mile “protection zone” and larger “surveillance zone” around the backyard farm, Agence France Presse reported.

The cases occurred on a farm near the Ukrainian border and were the first incidences of H5N1 detected in Europe in a year, according to the European Commission. The last detected case was in a wild duck shot in southern Germany in March 2009.